Identification of novel nuclear factors required for chloroplast gene expression and photosystem I assembly
نویسندگان
چکیده
Abbreviations µE microeinstein (1 E = 1 mol of photons) Å Ångstrom A 0 PSI primary electron acceptor composed of chlorophyll a A 1 PSI secondary electron acceptor composed of phylloquinone ATP adenosine 5′-triphosphate bp base pairs BSA bovine serum albumin cDNA complementary DNA CES control by epistasy of synthesis Ci Curie cpm counts per minute DNA deoxyribonucleic acid dNTPs deoxynucleoside triphosphates EDTA ethylenediaminetetraacetic acid EGTA ethylene
منابع مشابه
Post-transcriptional steps involved in the assembly of photosystem I in Chlamydomonas.
Assembly of the PSI (photosystem I) complex in eukaryotic photosynthetic organisms depends on the concerted interactions of the nuclear and chloroplast genetic systems. We have identified several nucleus-encoded factors of Chlamydomonas reinhardtii that are specifically required for the synthesis of the two large chloroplast-encoded reaction-centre polypeptides, PsaA and PsaB, of photosystem I ...
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The intricate assembly of photosystem I (PSI), a large multiprotein complex in the thylakoid membrane, depends on auxiliary protein factors. One of the essential assembly factors for PSI is encoded by ycf3 (hypothetical chloroplast reading frame number 3) in the chloroplast genome of algae and higher plants. To identify novel factors involved in PSI assembly, we constructed an epitope-tagged ve...
متن کاملY3IP1, a Nucleus-Encoded Thylakoid Protein, Cooperates with the Plastid-Encoded Ycf3 Protein in Photosystem I Assembly of Tobacco and Arabidopsis W OA
The intricate assembly of photosystem I (PSI), a large multiprotein complex in the thylakoid membrane, depends on auxiliary protein factors. One of the essential assembly factors for PSI is encoded by ycf3 (hypothetical chloroplast reading frame number 3) in the chloroplast genome of algae and higher plants. To identify novel factors involved in PSI assembly, we constructed an epitope-tagged ve...
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I Summary During evolution, most genes of the cyanobacterial ancestor of plastids were transferred to the nuclear genome of the host cell (endosymbiosis). Consequently, to maintain the physiological properties of plastids, new regulatory elements and protein import machineries have evolved. For proper assembly of the photosystem II complex, whose core proteins are still plastid encoded, several...
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